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KMID : 1134820180470070703
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Journal of the Korean Society of Food Science and Nutrition
2018 Volume.47 No. 7 p.703 ~ p.709
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Inhibitory Effect of Isorhamnetin on Lipid Accumulation in Free Fatty Acid-Induced Steatotic Hepatocytes through the PPARα Pathway
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Kim Chu-Sook
Cho Eun-Hee
Kang Min-Sook
Yu Ri-Na
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Abstract
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Obesity-induced hepatic lipid accumulation is a crucial factor in the initiation and development of non- alcoholic fatty liver disease. In this study, we investigated the effect of isorhamnetin, a naturally occurring flavonoid in fruits and vegetables, on free fatty acid (FFA)-induced lipid accumulation in hepatocytes. Hepatocytes (Hepa1c1c) were exposed to FFA with or without isorhamnetin, and lipid accumulation was estimated by Oil Red O staining and biochemical measurement. The metabolic genes/proteins involved in lipid metabolism were measured by qRT-PCR and/or western blotting. Isorhamnetin significantly reduced lipid accumulation in FFA-induced hepatocytes, and this was accompanied by modulation of genes involved in lipid metabolism. Isorhamnetin up-regulated expression of fatty acid oxidation-related genes (peroxisome proliferator-activated receptor α (PPARα), carnitine palmitoyltransferase 1, and acyl-CoA oxidase), and down-regulated lipogenic gene expression (sterol regulatory element-binding protein-1, fatty acid synthase, and acetyl-CoA carboxylase). Alterations in the metabolic gene expression induced by isorhamnetin were blunted by PPARα antagonist. These findings indicate that the inhibitory action of isorhamnetin in FFA-induced lipid accumulation in hepatocytes is associated with the PPARα pathway. Isorhamnetin may be useful for protection against obesity-related hepatosteatosis.
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KEYWORD
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obesity, fatty liver, hepatocyte, lipid metabolism, isorhamnetin
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